Partial purification of trypsin inhibitors from Parkia seeds (Fabaceae)

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41_2 T03_13_06_2014.indd

(Partial purification of trypsin inhibitors from Parkia seeds (Fabaceae)). Leguminous seeds (Fabaceae) have a high content of inhibitors of which serine protease inhibitors are the most widely studied. However, there are only a few studies related to the investigation of these proteins in tree species belonging to the Amazon flora. The protein content presented in seeds of four Amazonian Legumi...

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Purification and Partial Characterization of Trypsin-Specific Proteinase Inhibitors from Pigeonpea Wild Relative Cajanus platycarpus L. (Fabaceae) Active against Gut Proteases of Lepidopteran Pest Helicoverpa armigera

Proteinase inhibitors (PIs) are natural defense proteins of plants found to be active against gut proteases of various insects. A pigeonpea wild relative Cajanus platycarpus was identified as a source of resistance against Helicoverpa armigera, a most devastating pest of several crops including pigeonpea. In the light of earlier studies, trypsin-specific PIs (CpPI 63) were purified from mature ...

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Partial purification of the serum trypsin inhibitor.

The serum trypsin inhibitor has been studied by many investigators since Lansteiner (1) associated it with the albumin fraction by salt fractionation as did Smith and Lindsley (2) by the electrophoretic separation of serum. Both Christensen and MacLeod (3), and MacFarlane and Pilling (4), also associated the trypsin inhibitor with the albumin fraction but differentiated it from the plasmin inhi...

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Purification and Toxicity Study of a Saponin from Seeds of Albizia odorata, a Fabaceae from Madagascar

In order to continue the research of natural compounds of interest such as pesticides and therapeutic molecules in endemic species of Albizia from Madagascar, potentials of Albizia odorata seed extract were assessed. A toxic saponin (saponoside), named Albodorine, was isolated by extraction with hot ethanol or distilled water followed by purification procedure comprising n-butanol partition, pr...

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Genomic and cDNA cloning, expression, purification, and characterization of chymotrypsin-trypsin inhibitor from winged bean seeds.

A 516-bp winged bean chymotrypsin-trypsin inhibitor (WbCTI) gene was amplified from genomic DNA and cDNA isolated from winged bean using a pair of degenerate primers designed on the basis of the amino acid sequences of WbCTI. The amplified PCR products were cloned and sequenced to confirm their authenticity. DNA sequence analysis of the genomic and cDNA clones of WbCTI revealed the same nucleot...

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ژورنال

عنوان ژورنال: Hoehnea

سال: 2014

ISSN: 2236-8906

DOI: 10.1590/s2236-89062014000200003